Composite

Part:BBa_J45990

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2009-09-11)

Exponential phase expression of ATFI

A stationary phase promoter (the osmY promoter from E. coli) provides the transcription input signal to a transcriptional inverter (Q04401). The output of the inverter drives expression of the AtfI enzyme that converts isoamyl alcohol into isoamyl acetate (banana odor). The result should be exponential phase production of banana odor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1237
    Illegal BamHI site found at 2564
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1664
    Illegal SapI.rc site found at 2672


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